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mp 5444 bloxall endogenous peroxidase blocking solution vector laboratories  (Vector Laboratories)


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    Vector Laboratories mp 5444 bloxall endogenous peroxidase blocking solution vector laboratories
    Mp 5444 Bloxall Endogenous Peroxidase Blocking Solution Vector Laboratories, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 845 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+5444+bloxall+endogenous+peroxidase+blocking+solution+vector+laboratories/BLOXALL+Endogenous+Peroxidase+and+Alkaline+Phosphatase+Blocking+Solution/pmc07508173__mmc3-212-88-94
    Average 96 stars, based on 845 article reviews
    mp 5444 bloxall endogenous peroxidase blocking solution vector laboratories - by Bioz Stars, 2026-10
    96/100 stars

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    Recombinant:

    Article Title: Tumor-Promoting Ly-6G + SiglecF high Cells Are Mature and Long-Lived Neutrophils
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD45 (clone 30-F11) Biolegend Cat# 103126; RRID:AB_493535 Anti-Mouse CCR3 (clone J073E5) Biolegend Cat# 144510; RRID:AB_2561609 Anti-Mouse SiglecE (clone M1304A01) Biolegend Cat# 677106; RRID:AB_2566171 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109814; RRID:AB_389211 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109831; RRID:AB_10900256 Anti-Mouse CXCR2 (clone SA044G4) Biolegend Cat# 149304; RRID:AB_2565692 Anti-Mouse CD11b (clone M1/70) BD Biosciences Cat# 557657; RRID:AB_396772 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 551461; RRID:AB_394208 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 560599; RRID:AB_1727560 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127618; RRID:AB_1877261 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127602; RRID:AB_1089180 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127643; RRID:AB_2565971 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 564514; RRID:AB_2738833 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 552125; RRID:AB_394340 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 562681; RRID:AB_2722581 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 565526; RRID:AB_2739281 Anti-Mouse B220 (clone RA3-6B2) BD Biosciences Cat# 552772; RRID:AB_394458 Anti-Mouse CD90.2 (clone 53-2.1) BD Biosciences Cat# 561642; RRID:AB_10895975 Anti-Mouse CD45.1 (clone A20) BD Biosciences Cat# 560578; RRID:AB_1727488 Anti-Mouse CD16/32 (clone 93), TruStain FcX Biolegend Cat# 101320; RRID:AB_1574975 Biological Samples Lungs from KP1.9 tumor-bearing or tumorfree mice Mikael Pittet lab N/A Blood from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Spleens from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Bone marrow from KP1.9 tumor-bearing mice Mikael Pittet lab N/A (Continued on next page) Cell Reports 32, 108164, September 22, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bronchoalveolar lavage fluid from KP1.9 tumor-bearing or tumor-free mice Mikael Pittet lab N/A Chemicals, Peptides, and Recombinant Proteins 7-aminoactinomycin D Sigma Cat# A9400 Collagenase type I Worthington Cat# LS004197 ACK lysis buffer Lonza Cat# 10-548E Flow Cytometry Absolute Count Standard Bangs Laboratories Cat# 580 Histopaque-1077 Sigma-Aldrich Cat# 10771 Histopaque-1119 Sigma-Aldrich Cat# 11191 Critical Commercial Assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 Zombie Aqua Fixable Viability Kit Biolegend Cat# 423102 ImmPRESS-AP Anti-Rat IgG, Mouse Adsorbed (alkaline phosphatase) Polymer Detection Kit Vector Laboratories Cat# MP-5444 BLOXALL endogenous peroxidase blocking solution Vector Laboratories Cat# SP6000 DAB Quanto Chromogen and Substrate ThermoFisher Scientific Cat# TA-060-QHDX Deposited Data Neutrophil single cell RNA sequencing data (Engblom et al., 2017; Zilionis et al., 2019) GEO: GSE127465 (Engblom et al., 2017; Zilionis et al., 2019) Experimental Models: Cell Lines Murine KP1.9 lung adenocarcinoma cell line derived from lung tumor nodules of a C57BL/6 KrasLSL-G12D/WT;Trp53flox/flox mouse Alfred Zippelius N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J mice Jackson Laboratory Cat# 000664; RRID:IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ Jackson Laboratory Cat# 002014; RRID:IMSR_JAX:002014 Software and Algorithms FlowJo v.8.8.7 and 10.4 FlowJo, LLC RRID:SCR_008520 BD FACSDiva BD Biosciences RRID:SCR_001456 GraphPad Prism v.8 GraphPad Prism RRID:SCR_002798 Python 3.6.7 Anaconda https://www.anaconda.com/products/ individual NanoZoomer Digital Pathology (NDP.view2) Hamamatsu https://www.hamamatsu.com/eu/en/ product/type/U12388-01/index.html Aperio ImageScope Leica https://www.leicabiosystems.com/ digital-pathology/manage/ aperio-imagescope/ Scanpy v. 1.4.4.post1 (Wolf et al., 2018) https://scanpy.readthedocs.io/en/1.4.4. post1/ Report ll OPEN ACCESS ..

    Lysis:

    Article Title: Tumor-Promoting Ly-6G + SiglecF high Cells Are Mature and Long-Lived Neutrophils
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD45 (clone 30-F11) Biolegend Cat# 103126; RRID:AB_493535 Anti-Mouse CCR3 (clone J073E5) Biolegend Cat# 144510; RRID:AB_2561609 Anti-Mouse SiglecE (clone M1304A01) Biolegend Cat# 677106; RRID:AB_2566171 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109814; RRID:AB_389211 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109831; RRID:AB_10900256 Anti-Mouse CXCR2 (clone SA044G4) Biolegend Cat# 149304; RRID:AB_2565692 Anti-Mouse CD11b (clone M1/70) BD Biosciences Cat# 557657; RRID:AB_396772 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 551461; RRID:AB_394208 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 560599; RRID:AB_1727560 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127618; RRID:AB_1877261 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127602; RRID:AB_1089180 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127643; RRID:AB_2565971 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 564514; RRID:AB_2738833 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 552125; RRID:AB_394340 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 562681; RRID:AB_2722581 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 565526; RRID:AB_2739281 Anti-Mouse B220 (clone RA3-6B2) BD Biosciences Cat# 552772; RRID:AB_394458 Anti-Mouse CD90.2 (clone 53-2.1) BD Biosciences Cat# 561642; RRID:AB_10895975 Anti-Mouse CD45.1 (clone A20) BD Biosciences Cat# 560578; RRID:AB_1727488 Anti-Mouse CD16/32 (clone 93), TruStain FcX Biolegend Cat# 101320; RRID:AB_1574975 Biological Samples Lungs from KP1.9 tumor-bearing or tumorfree mice Mikael Pittet lab N/A Blood from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Spleens from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Bone marrow from KP1.9 tumor-bearing mice Mikael Pittet lab N/A (Continued on next page) Cell Reports 32, 108164, September 22, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bronchoalveolar lavage fluid from KP1.9 tumor-bearing or tumor-free mice Mikael Pittet lab N/A Chemicals, Peptides, and Recombinant Proteins 7-aminoactinomycin D Sigma Cat# A9400 Collagenase type I Worthington Cat# LS004197 ACK lysis buffer Lonza Cat# 10-548E Flow Cytometry Absolute Count Standard Bangs Laboratories Cat# 580 Histopaque-1077 Sigma-Aldrich Cat# 10771 Histopaque-1119 Sigma-Aldrich Cat# 11191 Critical Commercial Assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 Zombie Aqua Fixable Viability Kit Biolegend Cat# 423102 ImmPRESS-AP Anti-Rat IgG, Mouse Adsorbed (alkaline phosphatase) Polymer Detection Kit Vector Laboratories Cat# MP-5444 BLOXALL endogenous peroxidase blocking solution Vector Laboratories Cat# SP6000 DAB Quanto Chromogen and Substrate ThermoFisher Scientific Cat# TA-060-QHDX Deposited Data Neutrophil single cell RNA sequencing data (Engblom et al., 2017; Zilionis et al., 2019) GEO: GSE127465 (Engblom et al., 2017; Zilionis et al., 2019) Experimental Models: Cell Lines Murine KP1.9 lung adenocarcinoma cell line derived from lung tumor nodules of a C57BL/6 KrasLSL-G12D/WT;Trp53flox/flox mouse Alfred Zippelius N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J mice Jackson Laboratory Cat# 000664; RRID:IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ Jackson Laboratory Cat# 002014; RRID:IMSR_JAX:002014 Software and Algorithms FlowJo v.8.8.7 and 10.4 FlowJo, LLC RRID:SCR_008520 BD FACSDiva BD Biosciences RRID:SCR_001456 GraphPad Prism v.8 GraphPad Prism RRID:SCR_002798 Python 3.6.7 Anaconda https://www.anaconda.com/products/ individual NanoZoomer Digital Pathology (NDP.view2) Hamamatsu https://www.hamamatsu.com/eu/en/ product/type/U12388-01/index.html Aperio ImageScope Leica https://www.leicabiosystems.com/ digital-pathology/manage/ aperio-imagescope/ Scanpy v. 1.4.4.post1 (Wolf et al., 2018) https://scanpy.readthedocs.io/en/1.4.4. post1/ Report ll OPEN ACCESS ..

    Flow Cytometry:

    Article Title: Tumor-Promoting Ly-6G + SiglecF high Cells Are Mature and Long-Lived Neutrophils
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD45 (clone 30-F11) Biolegend Cat# 103126; RRID:AB_493535 Anti-Mouse CCR3 (clone J073E5) Biolegend Cat# 144510; RRID:AB_2561609 Anti-Mouse SiglecE (clone M1304A01) Biolegend Cat# 677106; RRID:AB_2566171 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109814; RRID:AB_389211 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109831; RRID:AB_10900256 Anti-Mouse CXCR2 (clone SA044G4) Biolegend Cat# 149304; RRID:AB_2565692 Anti-Mouse CD11b (clone M1/70) BD Biosciences Cat# 557657; RRID:AB_396772 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 551461; RRID:AB_394208 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 560599; RRID:AB_1727560 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127618; RRID:AB_1877261 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127602; RRID:AB_1089180 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127643; RRID:AB_2565971 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 564514; RRID:AB_2738833 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 552125; RRID:AB_394340 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 562681; RRID:AB_2722581 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 565526; RRID:AB_2739281 Anti-Mouse B220 (clone RA3-6B2) BD Biosciences Cat# 552772; RRID:AB_394458 Anti-Mouse CD90.2 (clone 53-2.1) BD Biosciences Cat# 561642; RRID:AB_10895975 Anti-Mouse CD45.1 (clone A20) BD Biosciences Cat# 560578; RRID:AB_1727488 Anti-Mouse CD16/32 (clone 93), TruStain FcX Biolegend Cat# 101320; RRID:AB_1574975 Biological Samples Lungs from KP1.9 tumor-bearing or tumorfree mice Mikael Pittet lab N/A Blood from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Spleens from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Bone marrow from KP1.9 tumor-bearing mice Mikael Pittet lab N/A (Continued on next page) Cell Reports 32, 108164, September 22, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bronchoalveolar lavage fluid from KP1.9 tumor-bearing or tumor-free mice Mikael Pittet lab N/A Chemicals, Peptides, and Recombinant Proteins 7-aminoactinomycin D Sigma Cat# A9400 Collagenase type I Worthington Cat# LS004197 ACK lysis buffer Lonza Cat# 10-548E Flow Cytometry Absolute Count Standard Bangs Laboratories Cat# 580 Histopaque-1077 Sigma-Aldrich Cat# 10771 Histopaque-1119 Sigma-Aldrich Cat# 11191 Critical Commercial Assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 Zombie Aqua Fixable Viability Kit Biolegend Cat# 423102 ImmPRESS-AP Anti-Rat IgG, Mouse Adsorbed (alkaline phosphatase) Polymer Detection Kit Vector Laboratories Cat# MP-5444 BLOXALL endogenous peroxidase blocking solution Vector Laboratories Cat# SP6000 DAB Quanto Chromogen and Substrate ThermoFisher Scientific Cat# TA-060-QHDX Deposited Data Neutrophil single cell RNA sequencing data (Engblom et al., 2017; Zilionis et al., 2019) GEO: GSE127465 (Engblom et al., 2017; Zilionis et al., 2019) Experimental Models: Cell Lines Murine KP1.9 lung adenocarcinoma cell line derived from lung tumor nodules of a C57BL/6 KrasLSL-G12D/WT;Trp53flox/flox mouse Alfred Zippelius N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J mice Jackson Laboratory Cat# 000664; RRID:IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ Jackson Laboratory Cat# 002014; RRID:IMSR_JAX:002014 Software and Algorithms FlowJo v.8.8.7 and 10.4 FlowJo, LLC RRID:SCR_008520 BD FACSDiva BD Biosciences RRID:SCR_001456 GraphPad Prism v.8 GraphPad Prism RRID:SCR_002798 Python 3.6.7 Anaconda https://www.anaconda.com/products/ individual NanoZoomer Digital Pathology (NDP.view2) Hamamatsu https://www.hamamatsu.com/eu/en/ product/type/U12388-01/index.html Aperio ImageScope Leica https://www.leicabiosystems.com/ digital-pathology/manage/ aperio-imagescope/ Scanpy v. 1.4.4.post1 (Wolf et al., 2018) https://scanpy.readthedocs.io/en/1.4.4. post1/ Report ll OPEN ACCESS ..

    Polymer:

    Article Title: Tumor-Promoting Ly-6G + SiglecF high Cells Are Mature and Long-Lived Neutrophils
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD45 (clone 30-F11) Biolegend Cat# 103126; RRID:AB_493535 Anti-Mouse CCR3 (clone J073E5) Biolegend Cat# 144510; RRID:AB_2561609 Anti-Mouse SiglecE (clone M1304A01) Biolegend Cat# 677106; RRID:AB_2566171 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109814; RRID:AB_389211 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109831; RRID:AB_10900256 Anti-Mouse CXCR2 (clone SA044G4) Biolegend Cat# 149304; RRID:AB_2565692 Anti-Mouse CD11b (clone M1/70) BD Biosciences Cat# 557657; RRID:AB_396772 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 551461; RRID:AB_394208 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 560599; RRID:AB_1727560 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127618; RRID:AB_1877261 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127602; RRID:AB_1089180 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127643; RRID:AB_2565971 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 564514; RRID:AB_2738833 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 552125; RRID:AB_394340 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 562681; RRID:AB_2722581 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 565526; RRID:AB_2739281 Anti-Mouse B220 (clone RA3-6B2) BD Biosciences Cat# 552772; RRID:AB_394458 Anti-Mouse CD90.2 (clone 53-2.1) BD Biosciences Cat# 561642; RRID:AB_10895975 Anti-Mouse CD45.1 (clone A20) BD Biosciences Cat# 560578; RRID:AB_1727488 Anti-Mouse CD16/32 (clone 93), TruStain FcX Biolegend Cat# 101320; RRID:AB_1574975 Biological Samples Lungs from KP1.9 tumor-bearing or tumorfree mice Mikael Pittet lab N/A Blood from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Spleens from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Bone marrow from KP1.9 tumor-bearing mice Mikael Pittet lab N/A (Continued on next page) Cell Reports 32, 108164, September 22, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bronchoalveolar lavage fluid from KP1.9 tumor-bearing or tumor-free mice Mikael Pittet lab N/A Chemicals, Peptides, and Recombinant Proteins 7-aminoactinomycin D Sigma Cat# A9400 Collagenase type I Worthington Cat# LS004197 ACK lysis buffer Lonza Cat# 10-548E Flow Cytometry Absolute Count Standard Bangs Laboratories Cat# 580 Histopaque-1077 Sigma-Aldrich Cat# 10771 Histopaque-1119 Sigma-Aldrich Cat# 11191 Critical Commercial Assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 Zombie Aqua Fixable Viability Kit Biolegend Cat# 423102 ImmPRESS-AP Anti-Rat IgG, Mouse Adsorbed (alkaline phosphatase) Polymer Detection Kit Vector Laboratories Cat# MP-5444 BLOXALL endogenous peroxidase blocking solution Vector Laboratories Cat# SP6000 DAB Quanto Chromogen and Substrate ThermoFisher Scientific Cat# TA-060-QHDX Deposited Data Neutrophil single cell RNA sequencing data (Engblom et al., 2017; Zilionis et al., 2019) GEO: GSE127465 (Engblom et al., 2017; Zilionis et al., 2019) Experimental Models: Cell Lines Murine KP1.9 lung adenocarcinoma cell line derived from lung tumor nodules of a C57BL/6 KrasLSL-G12D/WT;Trp53flox/flox mouse Alfred Zippelius N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J mice Jackson Laboratory Cat# 000664; RRID:IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ Jackson Laboratory Cat# 002014; RRID:IMSR_JAX:002014 Software and Algorithms FlowJo v.8.8.7 and 10.4 FlowJo, LLC RRID:SCR_008520 BD FACSDiva BD Biosciences RRID:SCR_001456 GraphPad Prism v.8 GraphPad Prism RRID:SCR_002798 Python 3.6.7 Anaconda https://www.anaconda.com/products/ individual NanoZoomer Digital Pathology (NDP.view2) Hamamatsu https://www.hamamatsu.com/eu/en/ product/type/U12388-01/index.html Aperio ImageScope Leica https://www.leicabiosystems.com/ digital-pathology/manage/ aperio-imagescope/ Scanpy v. 1.4.4.post1 (Wolf et al., 2018) https://scanpy.readthedocs.io/en/1.4.4. post1/ Report ll OPEN ACCESS ..

    Blocking Assay:

    Article Title: Tumor-Promoting Ly-6G + SiglecF high Cells Are Mature and Long-Lived Neutrophils
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD45 (clone 30-F11) Biolegend Cat# 103126; RRID:AB_493535 Anti-Mouse CCR3 (clone J073E5) Biolegend Cat# 144510; RRID:AB_2561609 Anti-Mouse SiglecE (clone M1304A01) Biolegend Cat# 677106; RRID:AB_2566171 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109814; RRID:AB_389211 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109831; RRID:AB_10900256 Anti-Mouse CXCR2 (clone SA044G4) Biolegend Cat# 149304; RRID:AB_2565692 Anti-Mouse CD11b (clone M1/70) BD Biosciences Cat# 557657; RRID:AB_396772 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 551461; RRID:AB_394208 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 560599; RRID:AB_1727560 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127618; RRID:AB_1877261 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127602; RRID:AB_1089180 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127643; RRID:AB_2565971 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 564514; RRID:AB_2738833 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 552125; RRID:AB_394340 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 562681; RRID:AB_2722581 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 565526; RRID:AB_2739281 Anti-Mouse B220 (clone RA3-6B2) BD Biosciences Cat# 552772; RRID:AB_394458 Anti-Mouse CD90.2 (clone 53-2.1) BD Biosciences Cat# 561642; RRID:AB_10895975 Anti-Mouse CD45.1 (clone A20) BD Biosciences Cat# 560578; RRID:AB_1727488 Anti-Mouse CD16/32 (clone 93), TruStain FcX Biolegend Cat# 101320; RRID:AB_1574975 Biological Samples Lungs from KP1.9 tumor-bearing or tumorfree mice Mikael Pittet lab N/A Blood from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Spleens from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Bone marrow from KP1.9 tumor-bearing mice Mikael Pittet lab N/A (Continued on next page) Cell Reports 32, 108164, September 22, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bronchoalveolar lavage fluid from KP1.9 tumor-bearing or tumor-free mice Mikael Pittet lab N/A Chemicals, Peptides, and Recombinant Proteins 7-aminoactinomycin D Sigma Cat# A9400 Collagenase type I Worthington Cat# LS004197 ACK lysis buffer Lonza Cat# 10-548E Flow Cytometry Absolute Count Standard Bangs Laboratories Cat# 580 Histopaque-1077 Sigma-Aldrich Cat# 10771 Histopaque-1119 Sigma-Aldrich Cat# 11191 Critical Commercial Assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 Zombie Aqua Fixable Viability Kit Biolegend Cat# 423102 ImmPRESS-AP Anti-Rat IgG, Mouse Adsorbed (alkaline phosphatase) Polymer Detection Kit Vector Laboratories Cat# MP-5444 BLOXALL endogenous peroxidase blocking solution Vector Laboratories Cat# SP6000 DAB Quanto Chromogen and Substrate ThermoFisher Scientific Cat# TA-060-QHDX Deposited Data Neutrophil single cell RNA sequencing data (Engblom et al., 2017; Zilionis et al., 2019) GEO: GSE127465 (Engblom et al., 2017; Zilionis et al., 2019) Experimental Models: Cell Lines Murine KP1.9 lung adenocarcinoma cell line derived from lung tumor nodules of a C57BL/6 KrasLSL-G12D/WT;Trp53flox/flox mouse Alfred Zippelius N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J mice Jackson Laboratory Cat# 000664; RRID:IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ Jackson Laboratory Cat# 002014; RRID:IMSR_JAX:002014 Software and Algorithms FlowJo v.8.8.7 and 10.4 FlowJo, LLC RRID:SCR_008520 BD FACSDiva BD Biosciences RRID:SCR_001456 GraphPad Prism v.8 GraphPad Prism RRID:SCR_002798 Python 3.6.7 Anaconda https://www.anaconda.com/products/ individual NanoZoomer Digital Pathology (NDP.view2) Hamamatsu https://www.hamamatsu.com/eu/en/ product/type/U12388-01/index.html Aperio ImageScope Leica https://www.leicabiosystems.com/ digital-pathology/manage/ aperio-imagescope/ Scanpy v. 1.4.4.post1 (Wolf et al., 2018) https://scanpy.readthedocs.io/en/1.4.4. post1/ Report ll OPEN ACCESS ..

    Single Cell:

    Article Title: Tumor-Promoting Ly-6G + SiglecF high Cells Are Mature and Long-Lived Neutrophils
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD45 (clone 30-F11) Biolegend Cat# 103126; RRID:AB_493535 Anti-Mouse CCR3 (clone J073E5) Biolegend Cat# 144510; RRID:AB_2561609 Anti-Mouse SiglecE (clone M1304A01) Biolegend Cat# 677106; RRID:AB_2566171 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109814; RRID:AB_389211 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109831; RRID:AB_10900256 Anti-Mouse CXCR2 (clone SA044G4) Biolegend Cat# 149304; RRID:AB_2565692 Anti-Mouse CD11b (clone M1/70) BD Biosciences Cat# 557657; RRID:AB_396772 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 551461; RRID:AB_394208 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 560599; RRID:AB_1727560 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127618; RRID:AB_1877261 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127602; RRID:AB_1089180 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127643; RRID:AB_2565971 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 564514; RRID:AB_2738833 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 552125; RRID:AB_394340 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 562681; RRID:AB_2722581 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 565526; RRID:AB_2739281 Anti-Mouse B220 (clone RA3-6B2) BD Biosciences Cat# 552772; RRID:AB_394458 Anti-Mouse CD90.2 (clone 53-2.1) BD Biosciences Cat# 561642; RRID:AB_10895975 Anti-Mouse CD45.1 (clone A20) BD Biosciences Cat# 560578; RRID:AB_1727488 Anti-Mouse CD16/32 (clone 93), TruStain FcX Biolegend Cat# 101320; RRID:AB_1574975 Biological Samples Lungs from KP1.9 tumor-bearing or tumorfree mice Mikael Pittet lab N/A Blood from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Spleens from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Bone marrow from KP1.9 tumor-bearing mice Mikael Pittet lab N/A (Continued on next page) Cell Reports 32, 108164, September 22, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bronchoalveolar lavage fluid from KP1.9 tumor-bearing or tumor-free mice Mikael Pittet lab N/A Chemicals, Peptides, and Recombinant Proteins 7-aminoactinomycin D Sigma Cat# A9400 Collagenase type I Worthington Cat# LS004197 ACK lysis buffer Lonza Cat# 10-548E Flow Cytometry Absolute Count Standard Bangs Laboratories Cat# 580 Histopaque-1077 Sigma-Aldrich Cat# 10771 Histopaque-1119 Sigma-Aldrich Cat# 11191 Critical Commercial Assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 Zombie Aqua Fixable Viability Kit Biolegend Cat# 423102 ImmPRESS-AP Anti-Rat IgG, Mouse Adsorbed (alkaline phosphatase) Polymer Detection Kit Vector Laboratories Cat# MP-5444 BLOXALL endogenous peroxidase blocking solution Vector Laboratories Cat# SP6000 DAB Quanto Chromogen and Substrate ThermoFisher Scientific Cat# TA-060-QHDX Deposited Data Neutrophil single cell RNA sequencing data (Engblom et al., 2017; Zilionis et al., 2019) GEO: GSE127465 (Engblom et al., 2017; Zilionis et al., 2019) Experimental Models: Cell Lines Murine KP1.9 lung adenocarcinoma cell line derived from lung tumor nodules of a C57BL/6 KrasLSL-G12D/WT;Trp53flox/flox mouse Alfred Zippelius N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J mice Jackson Laboratory Cat# 000664; RRID:IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ Jackson Laboratory Cat# 002014; RRID:IMSR_JAX:002014 Software and Algorithms FlowJo v.8.8.7 and 10.4 FlowJo, LLC RRID:SCR_008520 BD FACSDiva BD Biosciences RRID:SCR_001456 GraphPad Prism v.8 GraphPad Prism RRID:SCR_002798 Python 3.6.7 Anaconda https://www.anaconda.com/products/ individual NanoZoomer Digital Pathology (NDP.view2) Hamamatsu https://www.hamamatsu.com/eu/en/ product/type/U12388-01/index.html Aperio ImageScope Leica https://www.leicabiosystems.com/ digital-pathology/manage/ aperio-imagescope/ Scanpy v. 1.4.4.post1 (Wolf et al., 2018) https://scanpy.readthedocs.io/en/1.4.4. post1/ Report ll OPEN ACCESS ..

    RNA Sequencing:

    Article Title: Tumor-Promoting Ly-6G + SiglecF high Cells Are Mature and Long-Lived Neutrophils
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD45 (clone 30-F11) Biolegend Cat# 103126; RRID:AB_493535 Anti-Mouse CCR3 (clone J073E5) Biolegend Cat# 144510; RRID:AB_2561609 Anti-Mouse SiglecE (clone M1304A01) Biolegend Cat# 677106; RRID:AB_2566171 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109814; RRID:AB_389211 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109831; RRID:AB_10900256 Anti-Mouse CXCR2 (clone SA044G4) Biolegend Cat# 149304; RRID:AB_2565692 Anti-Mouse CD11b (clone M1/70) BD Biosciences Cat# 557657; RRID:AB_396772 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 551461; RRID:AB_394208 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 560599; RRID:AB_1727560 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127618; RRID:AB_1877261 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127602; RRID:AB_1089180 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127643; RRID:AB_2565971 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 564514; RRID:AB_2738833 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 552125; RRID:AB_394340 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 562681; RRID:AB_2722581 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 565526; RRID:AB_2739281 Anti-Mouse B220 (clone RA3-6B2) BD Biosciences Cat# 552772; RRID:AB_394458 Anti-Mouse CD90.2 (clone 53-2.1) BD Biosciences Cat# 561642; RRID:AB_10895975 Anti-Mouse CD45.1 (clone A20) BD Biosciences Cat# 560578; RRID:AB_1727488 Anti-Mouse CD16/32 (clone 93), TruStain FcX Biolegend Cat# 101320; RRID:AB_1574975 Biological Samples Lungs from KP1.9 tumor-bearing or tumorfree mice Mikael Pittet lab N/A Blood from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Spleens from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Bone marrow from KP1.9 tumor-bearing mice Mikael Pittet lab N/A (Continued on next page) Cell Reports 32, 108164, September 22, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bronchoalveolar lavage fluid from KP1.9 tumor-bearing or tumor-free mice Mikael Pittet lab N/A Chemicals, Peptides, and Recombinant Proteins 7-aminoactinomycin D Sigma Cat# A9400 Collagenase type I Worthington Cat# LS004197 ACK lysis buffer Lonza Cat# 10-548E Flow Cytometry Absolute Count Standard Bangs Laboratories Cat# 580 Histopaque-1077 Sigma-Aldrich Cat# 10771 Histopaque-1119 Sigma-Aldrich Cat# 11191 Critical Commercial Assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 Zombie Aqua Fixable Viability Kit Biolegend Cat# 423102 ImmPRESS-AP Anti-Rat IgG, Mouse Adsorbed (alkaline phosphatase) Polymer Detection Kit Vector Laboratories Cat# MP-5444 BLOXALL endogenous peroxidase blocking solution Vector Laboratories Cat# SP6000 DAB Quanto Chromogen and Substrate ThermoFisher Scientific Cat# TA-060-QHDX Deposited Data Neutrophil single cell RNA sequencing data (Engblom et al., 2017; Zilionis et al., 2019) GEO: GSE127465 (Engblom et al., 2017; Zilionis et al., 2019) Experimental Models: Cell Lines Murine KP1.9 lung adenocarcinoma cell line derived from lung tumor nodules of a C57BL/6 KrasLSL-G12D/WT;Trp53flox/flox mouse Alfred Zippelius N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J mice Jackson Laboratory Cat# 000664; RRID:IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ Jackson Laboratory Cat# 002014; RRID:IMSR_JAX:002014 Software and Algorithms FlowJo v.8.8.7 and 10.4 FlowJo, LLC RRID:SCR_008520 BD FACSDiva BD Biosciences RRID:SCR_001456 GraphPad Prism v.8 GraphPad Prism RRID:SCR_002798 Python 3.6.7 Anaconda https://www.anaconda.com/products/ individual NanoZoomer Digital Pathology (NDP.view2) Hamamatsu https://www.hamamatsu.com/eu/en/ product/type/U12388-01/index.html Aperio ImageScope Leica https://www.leicabiosystems.com/ digital-pathology/manage/ aperio-imagescope/ Scanpy v. 1.4.4.post1 (Wolf et al., 2018) https://scanpy.readthedocs.io/en/1.4.4. post1/ Report ll OPEN ACCESS ..

    Derivative Assay:

    Article Title: Tumor-Promoting Ly-6G + SiglecF high Cells Are Mature and Long-Lived Neutrophils
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mouse CD45 (clone 30-F11) Biolegend Cat# 103126; RRID:AB_493535 Anti-Mouse CCR3 (clone J073E5) Biolegend Cat# 144510; RRID:AB_2561609 Anti-Mouse SiglecE (clone M1304A01) Biolegend Cat# 677106; RRID:AB_2566171 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109814; RRID:AB_389211 Anti-Mouse CD45.2 (clone 104) Biolegend Cat# 109831; RRID:AB_10900256 Anti-Mouse CXCR2 (clone SA044G4) Biolegend Cat# 149304; RRID:AB_2565692 Anti-Mouse CD11b (clone M1/70) BD Biosciences Cat# 557657; RRID:AB_396772 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 551461; RRID:AB_394208 Anti-Mouse Ly-6G (clone1A8) BD Biosciences Cat# 560599; RRID:AB_1727560 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127618; RRID:AB_1877261 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127602; RRID:AB_1089180 Anti-Mouse Ly-6G (clone1A8) Biolegend Cat# 127643; RRID:AB_2565971 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 564514; RRID:AB_2738833 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 552125; RRID:AB_394340 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 562681; RRID:AB_2722581 Anti-Mouse SiglecF (clone E50-2440) BD Biosciences Cat# 565526; RRID:AB_2739281 Anti-Mouse B220 (clone RA3-6B2) BD Biosciences Cat# 552772; RRID:AB_394458 Anti-Mouse CD90.2 (clone 53-2.1) BD Biosciences Cat# 561642; RRID:AB_10895975 Anti-Mouse CD45.1 (clone A20) BD Biosciences Cat# 560578; RRID:AB_1727488 Anti-Mouse CD16/32 (clone 93), TruStain FcX Biolegend Cat# 101320; RRID:AB_1574975 Biological Samples Lungs from KP1.9 tumor-bearing or tumorfree mice Mikael Pittet lab N/A Blood from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Spleens from KP1.9 tumor-bearing mice Mikael Pittet lab N/A Bone marrow from KP1.9 tumor-bearing mice Mikael Pittet lab N/A (Continued on next page) Cell Reports 32, 108164, September 22, 2020 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Bronchoalveolar lavage fluid from KP1.9 tumor-bearing or tumor-free mice Mikael Pittet lab N/A Chemicals, Peptides, and Recombinant Proteins 7-aminoactinomycin D Sigma Cat# A9400 Collagenase type I Worthington Cat# LS004197 ACK lysis buffer Lonza Cat# 10-548E Flow Cytometry Absolute Count Standard Bangs Laboratories Cat# 580 Histopaque-1077 Sigma-Aldrich Cat# 10771 Histopaque-1119 Sigma-Aldrich Cat# 11191 Critical Commercial Assays Fixation/Permeabilization Solution Kit BD Biosciences Cat# 554714 Zombie Aqua Fixable Viability Kit Biolegend Cat# 423102 ImmPRESS-AP Anti-Rat IgG, Mouse Adsorbed (alkaline phosphatase) Polymer Detection Kit Vector Laboratories Cat# MP-5444 BLOXALL endogenous peroxidase blocking solution Vector Laboratories Cat# SP6000 DAB Quanto Chromogen and Substrate ThermoFisher Scientific Cat# TA-060-QHDX Deposited Data Neutrophil single cell RNA sequencing data (Engblom et al., 2017; Zilionis et al., 2019) GEO: GSE127465 (Engblom et al., 2017; Zilionis et al., 2019) Experimental Models: Cell Lines Murine KP1.9 lung adenocarcinoma cell line derived from lung tumor nodules of a C57BL/6 KrasLSL-G12D/WT;Trp53flox/flox mouse Alfred Zippelius N/A Experimental Models: Organisms/Strains Mouse: C57BL/6J mice Jackson Laboratory Cat# 000664; RRID:IMSR_JAX:000664 Mouse: B6.SJL-Ptprca Pepcb/BoyJ Jackson Laboratory Cat# 002014; RRID:IMSR_JAX:002014 Software and Algorithms FlowJo v.8.8.7 and 10.4 FlowJo, LLC RRID:SCR_008520 BD FACSDiva BD Biosciences RRID:SCR_001456 GraphPad Prism v.8 GraphPad Prism RRID:SCR_002798 Python 3.6.7 Anaconda https://www.anaconda.com/products/ individual NanoZoomer Digital Pathology (NDP.view2) Hamamatsu https://www.hamamatsu.com/eu/en/ product/type/U12388-01/index.html Aperio ImageScope Leica https://www.leicabiosystems.com/ digital-pathology/manage/ aperio-imagescope/ Scanpy v. 1.4.4.post1 (Wolf et al., 2018) https://scanpy.readthedocs.io/en/1.4.4. post1/ Report ll OPEN ACCESS ..



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